Protein Elution From Pvdf Membranes Using Detergents

Binding affinities of most proteins to PVDF membranes are relatively strong. The most efficient protocol for protein recovery from PVDF membranes requires the use of detergents, which limits the possible use of extracted samples because detergents are often incompatible with subsequent procedures. This protocol is a simple procedure to elute proteins from the membrane into a Triton/SDS solution. A protocol that employs acidic extraction with organic solvents is also described (Alternate Protocol 4).

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