Common Classical Preclinical In Vivo Approaches 702411 Subcutaneous or flank tumor models

In a typical murine tumor experiment, the cell line of interest is expanded in vitro and harvested at a point when the cells are in log growth. The number of inoculated cells needed for robust growth in vivo must be determined empirically and can vary from approximately 5 x 104 to 1 x 107, depending on the cell line. Important factors to consider when determining the appropriate cell titer include rate of tumor growth, variability in growth, and degree of tumor ulceration and necrosis. The strain of mice used (e.g., nude, scid, scid-bg for human or syngeneic for murine cell lines) can also significantly impact these parameters. Growth factors (usually in the form of Matrigel) are also often added to the cellular inoculum to enhance the initial in vivo growth phase. An n of 5 mice per treatment group is generally considered to be a minimum sample size, with a group size of 10 being preferred. Efficacy experiments are conducted either as early treatment studies (sometimes referred to as tumor growth inhibition) in which therapy is

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