Confocal Laser Scanning Microscopy Of Human Skin Fibroblasts Showing Transient Expression Of A Green Fluorescent

Roelofsen1, K. E. Niezen-Koning, E. A. A. Nollen2, and J. R. G. Kuipers Department of Pediatrics department of Internal Medicine Department of Radiobiology Groningen Utrecht Institute for Drug Exploration Groningen University, Groningen The Netherlands The mitochondrial outer membrane enzyme carnitine palmitoyltransferase 1 CPT1 is a main site of regulation of intracellular long-chain fatty acid transport. At least two isoforms of CPT1 are expressed in the body L-CPT1...

Recent Studies on the Possible Identity of Microsomal CATa

Although CATA can be solubilized from rat liver microsomes using mild detergents such as deoxycholate5 and reconstituted into liposomes of known phospholipid compo-sition10 without loss of activity, further purification of the enzyme away from other membrane components requires harsher conditions, which inactivate the enzyme. For example, substantial further purification can be achieved by extraction into 8mM CHAPS with 4 M urea followed by anion-exchange chromatography and gel filtration N. M....

Preface

In September 1991, Victor Zammit and I were in the Department of Biochemistry, the University of Cambridge, discussing our collaborative research project when we realized the potential value and need for a conference specifically concerned with fatty acid oxidation and ketogenesis. The idea, once seeded, was indulged and flourished into the first Fatty Acid Oxidation amp Ketogenesis FAOx amp K Conference that was held in the Department at Eastertime, 1992. It was attended by colleagues mainly...

References

McGarry, J.D., Woeltje, K.F., Kuwajima, M. amp Foster, D.W. 1989 Diabetes Metab. Rev., 5, 271-284. Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase. 2. Eaton, S., Bartlett, K. amp Pourfarzam, M. 1996 Biochem. J., 320, 345-357. Mammalian mitochondrial beta-oxidation. 3. McGarry, J.D. amp Brown, N.F. 1997 Eur. J. Biochem., 244, 1-14. The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. 4. Park, E.A. amp Cook, G.A. 1998 Mol....