Glucocorticoids and Fat Mobilisation a Response to Stress

We can more easily understand the logic of the synergistic interactions of the GR and PPAR RXR signal pathways on mHS gene expression, when we realise that one of the functions of glucocorticoid release into the blood is in the response of starvation stress.21 Starvation partly parallels the situations of the suckling neonate or the adult on a high fat low carbohydrate diet in that all three situations result in elevated plasma free fatty acid concentrations.22,23 In the case of starvation,...

Recent Studies on the Possible Identity of Microsomal CATa

Although CATA can be solubilized from rat liver microsomes using mild detergents such as deoxycholate5 and reconstituted into liposomes of known phospholipid compo-sition10 without loss of activity, further purification of the enzyme away from other membrane components requires harsher conditions, which inactivate the enzyme. For example, substantial further purification can be achieved by extraction into 8mM CHAPS with 4 M urea followed by anion-exchange chromatography and gel filtration N. M....

References

McGarry, J.D., Woeltje, K.F., Kuwajima, M. amp Foster, D.W. 1989 Diabetes Metab. Rev., 5, 271-284. Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase. 2. Eaton, S., Bartlett, K. amp Pourfarzam, M. 1996 Biochem. J., 320, 345-357. Mammalian mitochondrial beta-oxidation. 3. McGarry, J.D. amp Brown, N.F. 1997 Eur. J. Biochem., 244, 1-14. The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. 4. Park, E.A. amp Cook, G.A. 1998 Mol....