Thermal Stability Of The Wild Type And G242r Mcad

The thermal stability of the partially active MCAD mutants has so far been determined only on the cellular lysates after co-overexpression with GroEL and GroES.11,23 The G242R mutant has been shown to have a slightly reduced stability compared to the wild type. However, the presence of chaperonins and other proteins may have a significant influence on the results, so temperature stability of the purified proteins was also measured. The enzymes were incubated at 37 °C and residual activity was measured over a 100 minute period. The results are shown in figure 2.

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