Bivariate Analysis Of Redshifted Gfp For Cellcyclebasic

STUDIES PROTOCOL 2

This protocol describes the simultaneous detection of red-shifted GFP expression and DNA content revealed by propidium iodide (PI). Initially, the protocol may be used to address the question of whether the level of GFP expression per se may alter the distribution of cells within specific phases of the cell cycle in particular cell types. Once it has been established that GFP is not toxic, highly informative experiments may subsequently be performed in which expression of genes of interest fused to the GFP gene may be correlated with the cell cycle.

J J Studies of Cell

Function

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