Detection of Borreliacidal Antibodies by Flow Cytometry

unit 11.5

Borreliacidal antibodies are lethal to the spirochete Borrelia burgdorferi, the causative agent of Lyme disease. Detection of borreliacidal antibodies is useful for serodiagnosing Lyme disease and monitoring immune status after vaccination. Their detection, however, is dependent on the use of live organisms. Visual assessment of cell viability or monitoring of pH-dependent color changes in growth medium can be used to identify killing by borreliacidal antibodies, but detection by flow cytometry significantly increases sensitivity by allowing evaluation of small numbers of organisms. In addition, data from multiple assays can be rapidly acquired and analyzed objectively.

CAUTION: Live organisms are potentially pathogenic. Wear appropriate protective clothing and follow universal precautions.

NOTE: All solutions and equipment coming in contact with live cells must be sterile, and proper aseptic technique should be used accordingly.

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