Determining The Viability Of Yeast

Loss of the plasma membrane electrochemical potential provides an excellent indication of cell death in a population of yeasts (Dinsdale et al., 1995, 1999; Seward et al., 1996; Willetts et al., 1997). Flow cytometry provides the most satisfactory method for enumeration of dead cells after brief exposure of the organisms to the anionic voltage-sensitive oxonol dye bis-(1,3-dibutylbarbituric acid) trimethine oxonol, often referred to as DiBAC4(3). Viable cells exclude the fluorophore so that only dead cells become fluorescent.

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