How To Measure Fret Efficiency

The energy transfer efficiency, as follows from the above formulas, can be determined in a number of different ways. Since energy is transferred from the excited donor to the acceptor, the lifetime (t), quantum efficiency (Q), and fluorescence intensity (F) of the donor decrease, if the acceptor is present (Equation 1.12.10). As a consequence, the fluorescence

Energy Transfer Efficiency Distance
Figure 1.12.4 Distance dependence of the energy transfer efficiency. Distances are expressed in R0 units. The shaded area shows the useful distance range, where the energy transfer efficiency is between 0.95 and 0.05, meaning (0.61 x R0) - (1.63 x R0). Note that the curve is asymmetrical.

Flow Cytometry Instrumentation

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Responses

  • Jaime
    How to measure fret efficiency?
    6 years ago
  • samuel amanuel
    How to measure fret by flow cytometry?
    6 years ago

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