Preparation Of B Burgdorferi Stock Culture

The isolate of B. burgdorferi used to detect borreliacidal antibodies is critical. Borreliacidal antibodies will not be detected unless the spirochete expresses the homologous protein on the surface (Callister et al., 1996, 2002; Rousselle et al., 1998).

Materials

Borrelia burgdorferi

Barbour-Stoenner-Kelly (BSK) medium (see Support Protocols 1 and 2) 13 x 100-mm sterile culture tubes

1. Inoculate 200 to 500 ^l B. burgdorferi organisms into 6 ml fresh BSK medium per 13 x 100-mm sterile culture tube.

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