Reagents And Solutions

Use sterile deionized, distilled water in all recipes and protocol steps. For common stock solutions, see APPENDIX 2; for suppliers, see SUPPLIERS APPENDIX.

Cryopreservation solution

15 ml dimethyl sulfoxide (DMSO; 15% final)

H2O to 100 ml

Propidium iodide buffer 250 ml H2O

0.2541 g MgCl26H2O (5 mM final)

0.0125 g propidium iodide (0.05 mg/ml final)

Adjust pH to near 7.0 with concentrated HCl

Adjust pH to exactly 7.0 with 1 M HCl

Store up to 3 weeks at 4°C in a foil-wrapped container

Handling, Storage, and Preparation of Human Tissues

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