Ludwig Eichinger Francisco Rivero

Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development Time-lapse microscopy provides a powerful tool with which to study cell behavior during Dictyostelium development. On a macroscopic level, the overall cell movement patterns that give rise to the complex multicellular structures such as slugs and fruiting bodies can be studied together with the signal waves that coordinate cell movement. Using green fluorescent protein fusion proteins, it is...

Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells

Grafix Biochemical

Neuhaus, and Thierry Soldati Summary Recent years have seen a powerful revival of fluorescence microscopy techniques, both to observe live cells and fixed objects. The limits of sensitivity, simultaneous detection of multiple chromophores, and spatial resolution have all been pushed to the extreme. Therefore, it is essential to improve in parallel the quality of the structural and antigenic preservation during fixation and immunostaining. Chemical fixations are broadly...

The Secret Lives of Dictyostelium

Dictyostelium Nc4

Research on Dictyostelium has a long history, the trend of which has been to add cell biology, natural products, and evolutionary biology research to the traditional studies of development. The methods presented here and the recent publication of the genome present vast new opportunities. Key Words Cellular slime mold natural products soil ameba amoeboid genome. In 1991, the archives of the Soviet Union and its satellites began to open and to inform us in the West about events that we had...