Notes

The authors intend to use this section to draw the reader's attention to several additional features of the StroCDB, in particular, the pages of the database that have not been discussed yet. These pages provide background information, additional methods, supplemental biological data, and interactive tables.

1. The Introduction button on the home page of StroCDB will retrieve http://stromalcell. princeton.edu/files/intro.html, a page that provides a highly interactive perspective on stem cell biology in vivo and in vitro. Topical subsections of the discussion include the functional properties and origins of HSC, their physical characterization and purification, in vitro cytokine supported systems, stromal cell supported systems, defining the stem cell niche, the plasticity of stem cells, and the molecular definition of a stem cell niche. The various links embedded within the text retrieve PubMed references and additional primary data generated in our laboratory.

2. Additional methods used in these studies but not addressed above are available by clicking on the Methods button of the home page (http://stromalcell.princeton.edu/files/ methods.html). This page details methods for the generation of the stromal cell lines, stem cell purification strategies for both fetal liver and adult marrow, stem cell/stromal cell co-cultivation, in vitro hematopoietic progenitor assays, and in vivo transplantation assays for stem cell activity. Again, embedded within the text are links for various references and primary data.

3. Supplementary molecular and biological data are available at http://stromalcell.princeton. edu/files/supportive.html by clicking on the Supplementary Data button on the home page. Links on this page include but are not limited to: 1) experimental design of single cell deposition studies; 2) the developmental potential of single cells deposited on AFT024 cells; 3) reverse-transcriptase polymerase chain reaction expression data of common regulatory cytokines; 4) a retroviral marking strategy; 5) proviral integration data after marking on AFT024 cells; 6) Northern blot expression screen of clones from the library; and 7) cluster analyses from microarray experiments. Also included are studies with highly purified quiescent adult marrow and twice purified fetal liver stem cells.

4. The Figures and Tables button on the home page of StroCDB (http://stromalcell. princeton.edu/files/figures.html) provides access to interactive and expanded figures and tables presented in Hackney et al (12). These include two web-only tables: one for known secreted, cell surface, matrix and cytoskeletal proteins in StroCDB and the other for molecules regulating both neuronal and hematopoietic systems.

5. A major advantage to a web-based tool like the StroCDB is that it can be continually upgraded and refined. As new data become available, they can be easily integrated into the existing format or the format can be revised to reflect the changing needs of the investigator. We plan to add additional pages to the database that will provide links to a microarray platform under development for array data deposition, analyses and dissemination. We intend to deposit all the primary data related to the existing, published array experiments, including additional information related to the experiments.

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