Purification Of IgM By Ammonium Sulfate Precipitation

This protocol can be used for most IgM antibodies. It is more time-consuming and tedious than dialysis against water, but should be used for IgM antibodies that will not precipitate in water. Ammonium sulfate precipitation of IgM is similar to that described for IgG in unit2.7 Basic Protocol 1 . This is followed by dialysis and size-exclusion chromatogra-phy as described above for IgM. Additional Materials also see Basic Protocol 1 Saturated ammonium sulfate SAS unit 2.7 Ammonium sulfate...