In Vivo Tumor Protection And Immunotherapy Experiments

Vaccination Is Not Immunization Vaccine Risks Exposed

Vaccines Have Serious Side Effects

Get Instant Access

In general, vaccine formulations can be tested either for their ability to elicit anti-tumor protection or for eradication of established tumor. Although, the latter is the more challenging task, both assays are feasible using 38c-13 and A20 lymphomas. However, use of the slower-growing lymphoma, A20, is recommended for immunotherapy experiments. Both types of in vivo experiments, tumor protection and therapy of established tumor, are described in this protocol. Moreover, the protocol starts with description of immunization strategies for tumor protection experiments using DNA ("a" steps), protein ("b" steps), and cytokine-transduced vaccines ("c" steps), followed by tumor challenge protocols, including a tumor challenge protocol for protection studies ("d" step; same as tumor challenge steps in Basic Protocol 1) and a detailed experiment for immunotherapy of established A20 lymphoma ("e" steps). All in vivo experiments should be repeated at least three times with 10 mice per experimental group (6- to 12-week-old syngeneic female mice). Ig-KLH, whole-cell, or chemokine-sFv fusion protein vaccines require immunization once or twice every two weeks, while DNA vaccines require three or more immunizations every two weeks. Sera (200 |l) are drawn from the retro-orbital spaces of five randomly selected mice per group before each boost and tested for the presence of anti-idiotypic antibodies by ELISA (Support Protocol 2), to monitor immunization, prior to tumor challenge.

Was this article helpful?

0 0
How To Bolster Your Immune System

How To Bolster Your Immune System

All Natural Immune Boosters Proven To Fight Infection, Disease And More. Discover A Natural, Safe Effective Way To Boost Your Immune System Using Ingredients From Your Kitchen Cupboard. The only common sense, no holds barred guide to hit the market today no gimmicks, no pills, just old fashioned common sense remedies to cure colds, influenza, viral infections and more.

Get My Free Audio Book

Post a comment