Induction Of Airway Hyperresponsiveness By Systemic Sensitization And Airway Challenge With Ovalbumin

This section describes the most widely used method to induce AHR, repeated systemic sensitization followed by subsequent airway challenge with the same antigen. In this way sensitization and airway provocation with allergen are separated and can be manipulated individually. Repeated injection of allergen mixed in aluminum hydroxide (alum) as an adjuvant induces strong, sustained sensitization with a preferential TH2-type immune response and production of allergen-specific IgE and IgG1 (Beck and Spiegelberg, 1989). Airway provocation of presensitized mice with the same allergen induces airway inflammation with preferential eosinophilic infiltration and, as a consequence, AHR. Control

Contributed by Eckard Hamelmann and Erwin W. Gelfand

Current Protocols in Immunology (1999) 15.18.1-15.18.13 Copyright © 1999 by John Wiley & Sons, Inc.

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