Insulin Therapy For Diabetic Rats

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Two methods of insulin therapy are described: daily subcutaneous injections of insulin for short-term maintenance and subcutaneous implantation of timed-release pellets of bovine insulin for long-term maintenance. The goal of treatment is not to produce a normal blood glucose concentration, as such "tight" control poses the risk of death from hypoglycemia, but rather to maintain growth and prevent ketoacidosis for the duration of the experiment. The dose of insulin is chosen on this basis. It is not necessary to monitor blood glucose concentrations in treated animals unless this parameter is a variable in an experimental study.

Materials

Diabetic rats (see Basic Protocol 1 or Alternate Protocol 1 or 2) Glucose reagent strips (Clinistix, Miles Labs)

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