Maintenance And Diagnosis Of Spontaneous Iddm In Diabetesprone Bb Rats

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This protocol describes the procurement and husbandry of BB rats, diagnosis of insulin-dependent diabetes mellitus (IDDM), treatment and care of diabetic animals, and his-topathological evaluation of insulitis. The methods in this protocol are common to all the models of IDDM in this unit. Diabetes is diagnosed clinically by documenting a sustained increase in blood glucose concentration. Insulitis invariably precedes the development of diabetes, but does not always progress to diabetes. Insulitis is diagnosed morphologically by assessment of mononuclear cell infiltration of pancreatic islets (see Support Protocol 1). Overt hyperglycemia occurs only when >90% of the P cell mass in the pancreas has been destroyed.

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