This protocol describes the procurement and husbandry of BB rats, diagnosis of insulin-dependent diabetes mellitus (IDDM), treatment and care of diabetic animals, and his-topathological evaluation of insulitis. The methods in this protocol are common to all the models of IDDM in this unit. Diabetes is diagnosed clinically by documenting a sustained increase in blood glucose concentration. Insulitis invariably precedes the development of diabetes, but does not always progress to diabetes. Insulitis is diagnosed morphologically by assessment of mononuclear cell infiltration of pancreatic islets (see Support Protocol 1). Overt hyperglycemia occurs only when >90% of the P cell mass in the pancreas has been destroyed.
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All you need is a proper diet of fresh fruits and vegetables and get plenty of exercise and you'll be fine. Ever heard those words from your doctor? If that's all heshe recommends then you're missing out an important ingredient for health that he's not telling you. Fact is that you can adhere to the strictest diet, watch everything you eat and get the exercise of amarathon runner and still come down with diabetic complications. Diet, exercise and standard drug treatments simply aren't enough to help keep your diabetes under control.