Vaginal Candidiasis In Mice

Vaginitis in rodents is inducible under conditions of pseudoestrus. Mice given a vaginal inoculum of C. albicans under these conditions acquire a persistent vaginal infection, while mice inoculated in the absence of estrogen resolve the infection within 3 weeks. This protocol describes vaginal candidiasis in estrogenized CBA/J mice, a model of infection particularly suitable for studies on local and peripheral immune reactivity occurring in this infection.


Female CBA/J (H-2a) mice, 8 to 10 weeks of age (Charles River) Estradiol valerate (Sigma) dissolved in sesame oil immediately before use C. albicans blastospores, 3153 serotype A (3153A; ATCC) PBS (appendix 2), sterile

Sabouraud's dextrose agar (see recipe): 9-cm plates containing 50 pg/ml chloramphenicol

Additional reagents and equipment for subcutaneous injection (unit 1.6), preparation of Candida suspension (see Support Protocol 1), and PAS staining (see Support Protocol 3)

How To Cure Yeast Infection

How To Cure Yeast Infection

Now if this is what you want, you’ve made a great decision to get and read this book. “How To Cure Yeast Infection” is a practical book that will open your eyes to the facts about yeast infection and educate you on how you can calmly test (diagnose) and treat yeast infection at home.

Get My Free Ebook

Post a comment