FIG. 10 Possible interaction mechanisms between immobilized PEI and DNA. (Reprinted with permission from Ref. 23, Copyright © 2000, Elsevier Science.)
of N-3-dimethylaminopropylmethacrylamide (DMAPM) . The variation of equilibrium DNA adsorption capacity with the initial DNA concentration at pH 7.4 and 25°C is shown in Fig. 13. As seen in the figure, the equilibrium DNA adsorption capacities up to 55 mg DNA/g dry beads could be achieved via the ionic interaction between the dimethylaminopropyl groups of the support material and phosphate bonds of DNA . In the desorption experiments, yields lower than 5% (w/w) were obtained in Tris-HCl buffer solution including 1 M
PEI attached-PCMS particles
^^ Bases in DNA D' Deoxyribose units
™ Phosphate bonds
- PEI chain
NaCl . These results indicated that the ionic interaction was sufficiently strong to obtain a stable DNA binding on the surface of the gel beads .
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