Pharmacology Methods of Analysis

Gas chromatography allows the measurement of chloroquine and its metabolite monodesethylchloroquine in concentrations of 30-60 |g/l (0.1-0.2 |imol/l). High performance liquid chromatography detects plasma concentrations of 3ng/l (10nmol/l) with a UV detector and of 0.15ng/ml (0.5nmol/l) with a fluorescence detector (Minzi et al. 2003, Walker and Ademowo 1996). The determination of chloroquine concentrations in saliva are not suited for the monitoring of therapy. Such monitoring is possible using hair and gas chromatography/mass spectrometry (Runne et al. 1992).

Because of the strong binding of chloroquine and its main metabolite to thrombocytes and granulocytes as well as the accumulation in erythrocytes (factors 2-5), the concentration in whole blood is 3-10 times higher than that in plasma. Compared with plasma, the chloroquine serum concentrations are higher and more variable because of the release of chloroquine out of thrombocytes during coagulation. To prevent these errors, adequate centrifugation (2000 g, 10-15 min) is required. The binding of chloroquine to glass may lower chloroquine concentrations in standard solutions and analytical jars. The addition of serum prevents this.

Hair Loss Prevention

Hair Loss Prevention

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