Na

Methotrexate/folate pQE vector set (Qiagen)

mAb/low pH

Purification and immobilization

Increased expression

E2-Tagging and Detection Kit (Abeam), E2 tagging technology (Quattromed)

Detection and purification

Typically used in conjunction with self-splicing Terpe (2003) intein tag; one-step purification of nearly 100% pure protein with low milligram yields; matrix compatible with high salt and nonionic detergents; purification must be done in absence of thiol-containing reducing agents until elution step; fusion protein may have effect on intein cleavage efficiency N-term CBD can immobilize fusion protein on choline-coated gold chip for SPR or microscopy studies; large tag or C-term tag placement may affect fusion properties May increase proteolytic stability of fusion proteins to increase expression; little immunogenicity in mouse and rat, therefore, useful for generation of antibodies to fusion; methotrexate purification does not give high yields; usually coupled with a short affinity tag (e.g., His-tag, Strep-tag) for purification

Derived from bovine papilloma-virus type-1 Kaldalu et al.

transactivator protein E2; primary antibodies (2000)

available for colorimetric and chemiluminescent detection; antibody purification does not give high yields, low pH

elution may irreversibly affect protein properties; matrix is of limited reusability

Morandi et al. (1984)

Length Position Matrix/elution Commercial Typical use(s)

(sequence) systems (supplier)

Comments

References

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