Methotrexate/folate pQE vector set (Qiagen)

mAb/low pH

Purification and immobilization

Increased expression

E2-Tagging and Detection Kit (Abeam), E2 tagging technology (Quattromed)

Detection and purification

Typically used in conjunction with self-splicing Terpe (2003) intein tag; one-step purification of nearly 100% pure protein with low milligram yields; matrix compatible with high salt and nonionic detergents; purification must be done in absence of thiol-containing reducing agents until elution step; fusion protein may have effect on intein cleavage efficiency N-term CBD can immobilize fusion protein on choline-coated gold chip for SPR or microscopy studies; large tag or C-term tag placement may affect fusion properties May increase proteolytic stability of fusion proteins to increase expression; little immunogenicity in mouse and rat, therefore, useful for generation of antibodies to fusion; methotrexate purification does not give high yields; usually coupled with a short affinity tag (e.g., His-tag, Strep-tag) for purification

Derived from bovine papilloma-virus type-1 Kaldalu et al.

transactivator protein E2; primary antibodies (2000)

available for colorimetric and chemiluminescent detection; antibody purification does not give high yields, low pH

elution may irreversibly affect protein properties; matrix is of limited reusability

Morandi et al. (1984)

Length Position Matrix/elution Commercial Typical use(s)

(sequence) systems (supplier)



Was this article helpful?

0 0
Healthy Chemistry For Optimal Health

Healthy Chemistry For Optimal Health

Thousands Have Used Chemicals To Improve Their Medical Condition. This Book Is one Of The Most Valuable Resources In The World When It Comes To Chemicals. Not All Chemicals Are Harmful For Your Body – Find Out Those That Helps To Maintain Your Health.

Get My Free Ebook

Post a comment