Pyridylethylation for Quantification of Cysteine

Alkylation of cysteine with 4-vinylpyridine produces pyridylethylcysteine, which is stable to acid hydrolysis (Friedman et al., 1970) and readily measured by PTC-AAA. Pyridylethylcysteine (Pec) is commercially available (Pierce) and can be used to determine an appropriate Pec response factor as described for other standard amino acids. The following protocol is adapted from Hawke and Yuan (1987).

Additional Materials (also see Basic Protocol)

1:3 (v/v) 1 M TrisHCl (pH 8.5) containing 4 mM EDTA (Sigma)/8 M guanidineHCl or 1 M TrisHCl/1 mM EDTA, pH 8.5, containing 8 M urea (ultrapure)

1 M TrisHCl, pH 8.5, or 1 N HCl, for adjusting pH if necessary

Was this article helpful?

0 0
Healthy Chemistry For Optimal Health

Healthy Chemistry For Optimal Health

Thousands Have Used Chemicals To Improve Their Medical Condition. This Book Is one Of The Most Valuable Resources In The World When It Comes To Chemicals. Not All Chemicals Are Harmful For Your Body – Find Out Those That Helps To Maintain Your Health.

Get My Free Ebook

Post a comment