Adverse Effects Interactions and Pharmacokinetics

Dosages of up to 30 mg rhDNase I per day were well tolerated in healthy volunteers and CF patients 69,87 . Severe bronchospasms or anaphylactic reactions, as seen after inhalation of bovine DNase I, have never been observed with rhDNase I. The most common adverse effects reported after daily inhalation of 2.5 mg rhDNase I were voice alterations hoarseness , pharyngitis, rash, laryngitis, and conjunctivitis 74,76,78 . All these events are generally mild and transient. In patients with severe...

References

Kreuder, V., et al., Isolation, characterization and crystallization of deoxyribonuclease-I from bovine and rat parotid-gland and its interaction with rabbit skeletal-muscle actin, Eur. J. Biochem., 139, 389-400, 1984. 2. Shiokawa, D. and Tanuma, S., Characterization of human DNase I family endonucleases and activation of DNase gamma during apoptosis, Biochemistry, 40, 143-152, 2001. 3. Evans, C.J. and Aguilera, R.J., DNase II Genes, enzymes and function, Gene, 322, 1-15, 2003. 4. Nadano,...

Nase I in NonCF Respiratory Diseases

Highly viscoelastic lung mucus causes many other respiratory disorders like atelectasis, bronchiectasis, bronchiolitis, bronchitis, and primary ciliary dyskinesia PCD , giving rise to suffering and even morbidity. Under these conditions, the high viscidity of the lung secretions is often also due to high DNA concentrations, a result of a neutrophil-dominated airway inflammation. Consequently, rhDNase I may be of benefit to these patients. However, unlike the situation in CF, only a few...

Rasburicase Expression And Purification

8.2 Biochemical Characteristics of the 8.2.1 Structure 8.2.2 Kinetic 8.2.3 Related 8.3 Indications for which the Enzyme is 8.3.1.1 Mechanism of Uric Acid 8.3.1.2 Consequences of 8.3.2 Medical Conditions Requiring Uricolytic 8.3.2.2 Diseases and Causes of Acute 8.4 Developmental History of the Enzyme 8.4.1.1 Mechanism of 8.4.2 Safety of 8.4.3 Results of Rasburicase 8.5 Characteristics of the Enzyme's Producer Cell 8.5.1 The Expression Vector 8.5.1.1 The Urate Oxidase-Encoding 8.5.2 The Host...